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Solution 12 DAPI (4’,6-diamidino-2-phenylindole) 1ml

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SKU: 910-3012

Manufacturer: ChemoMetec

Availability: Order only

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Fluorescent dye - DAPI (4,6-Diamidino-2-phenylindole dihydrochloride).

Binds to double-stranded DNA of cells without intact membranes (dead cells).

Used in conjunction with solutions 10 and 11 in "Two-Step Cell Cycle Assay" in assays using the NC-250 and NC-3000, and for the assay of aggregated cells.


Description

Description

DAPI is a blue fluorescent dye that fluoresces brightly when selectively bound to the minor groove of double-stranded DNA, where its fluorescence is about 20 times higher than unbound. Its selectivity for DNA and high cell permeability allow efficient staining of nuclei with a low cytoplasmic background. DAPI is a classic nuclear contrast agent for immunofluorescence microscopy and is an important component of screening methods that require cell-based DNA quantification.

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