Solution 6 VB-48 propidium iodide (PI) 1 ml
SKU: 910-3006
Manufacturer: ChemoMetec
Availability: Order only
Solution 6 contains propidium iodide, which stains dead cells. In turn, VB-48 is a patented fluorophore that only fluoresces when bound to reduced thiols.
Used for measurements with the NucleoCounter 250 for viability analysis.
Description Recommended products
Description
Description
Viability Assay with NucleoCounter NC-250 System
The programmed method for the NucleoCounter NC-250 system allows the user to detect changes in the intracellular level of (reduced) thiols. Such changes can occur in apoptotic cells or cells undergoing other pathological processes. Since intracellular regenerative capacity is an indicator of overall health, this test provides a very simple and quick way to assess cell viability.
The cells to be tested are mixed with Solution 6. The solution contains two different reagents: propidium iodide (PI) for staining dead cells and VB-48, which stains viable cells depending on the intensity, which is dependent on the level of thiols in them. The high fluorescence intensity of a particular cell indicates that the cell has a high level of thiols such as GSH (glutathione).
The stained cells are immediately loaded onto NC-Slides: either 2-chamber NC-Slide A2 or 8-chamber NC-Slide A8. Samples are analyzed using the NucleoCounter NC-250 system. A fluorescence intensity histogram shows the distribution of thiol levels in all cells is displayed on the PC screen. By comparing histograms of treated cells with controls, a fraction of cells with low viability (eg, apoptotic or stressed cells) can be determined.
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Solution 11 Stabilization buffer, 100 ml
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Solution 5 VB-48 PI-AO (propidium iodide - acridine orange)
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Solution 20 VitaBright-48, 100 μl
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Solution 13 AO - DAPI 1ml
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Solution 17 Blood lysis buffer, 25 ml
Used in determining the viability and concentration of leukocytes in samples containing red erythrocytes.
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Solution 10 Lysis buffer, 100 ml
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Lysis / Dilution Buffer - Reagent S100
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